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Laboratorio di ricerca

Lab. nameGenomic and transcriptomic lab for Regenerative Medicine 
Principal Investigator Rossella Manfredini 
ComponentsRossella Manfredini  (Principal Investigator )
Description1. Molecular characterization of stem cells of squamous epithelia (epidermis, cornea, conjunctiva, urethra and buccal mucosa) and hematopoietic stem cells and identification of molecular mechanisms underlying their self-renewal, proliferation and differentiation. 2. Molecular and functional characterization of leukemic stem cells;  
Addressvia Giuseppe Campi, 287 - 41125 Modena 
KeywordsStem cells, genomics, transcriptomics, functional genomics,hematopoietical malignancies 
ERC Sectors 
Instrumentation1. n° 2 Affymetrix Platforms (GeneChip 3000, Affymetrix Fluidics Station 450, Affymetrix hybridization Oven 645 and GeneAtlasTM Personal Microarray System Affymetrix), Bioanalyzer 2100 (Agilent) "Lab-on-a-chip" platform, Roche (454) Genome Sequencer FLX titanium System, Ion Torrent PGM Sequencer (Life Technologies) 2. Nucleofector 4D device (Amaxa).
 3. FACS Canto II (BD) flow-cytometer (3 lasers). 4. Glomax 96 Microplate Luminometer (Promega) and High-Throughput RT-PCR systems (7900HT Fast Real Time PCR System and QuantStudio 12K Flex Real-Time PCR System and OpenArray AccuFill System, Applied Biosystems). 5. 6 workstations equipped with Linux, Windows or Macintosh operating systems and packages open-source for data analysis.
 
Research Lines Translational Medicine

Methodologies 1. Nucleic acid extraction and gene expression profiling by means of Affymetrix platforms, 2. Next generation sequencing, 3. cell culture and differentiation assays for hematopoietic stem cells, 4. Flow Cytometric analysis of cell cycle and of surface or intracellular markers, 5. gene silencing and overexpression, 6.bioinformatic analysis, 7. mouse model of disease (expecially leukemia mouse models), mice handling techniques and BM transplant (syngenic and xenogenic) 
Web Site www.cmr.unimore.it/home.html