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Laboratorio di ricerca

Lab. nameMolecular Biology of Retinal Degenerations 
Principal Investigator Valeria Marigo 
ComponentsValeria Marigo  (Principal Investigator )
Clara La Marca  (Dottorando )
Davide Schiroli  (Assegnista )
Antonella Comitato  (Collaboratore )
DescriptionThe laboratory of Molecular Biology of Retinal Degenerations focuses on molecular mechanisms underlying genetic diseases that impair vision: retinitis pigmentosa (RP). We apply molecular biology, biochemistry and cell biology techniques to identify molecular pathways in murine models of human diseases of the eye. We characterized molecular mechanisms underlying rod cells death caused by mutations in the PDE6B and RHO genes and demonstrated the key function of calpains, Bax and Aif. We are now interested in testing in vivo therapeutic molecules to counteract the degenerative process: 1) blockers of the apoptotic pathway such as cGMP analogs, calpain inhibitors, molecular chaperones for RHO 2) neurotrophic factors such as PEDF. We are also assessing cell therapy approaches for retinal degenerations affecting the retinal pigment epithelium (RPE). To this aim we are developing in vitro protocols to differentiate functional RPE from human retinal stem cells. 
Addressvia Giuseppe Campi, 287 - 41125 Modena 
KeywordsPhotoreceptor, Apoptosis, Cellular models, Rhodopsin, PEDF 
ERC Sectors LS1_1 Molecular interactions LS1_1 Molecular interactions LS1_11 Biochemistry and molecular mechanisms of signal transduction LS2_6 Molecular genetics, reverse genetics and RNAi LS3_4 Apoptosis LS3_8 Signal transduction LS5_4 Sensory systems (e.g. visual system, auditory system)  
Instrumentation2 termocilatori per PCR 1 Chemidoc Biorad per analisi di western blot e gel di agarosio 1 Microtomo Leica 1 Microscopio a Fluorescenza Zeiss 1 Stereomicroscopio a Fluorescenza Leica MZ16F 1 Stereomicroscopio Leica MZ75 1 Stereomicroscopio Leica 2 PC  
Research Lines Multi-scale and cell-based approaches to genetic and chronic disorders and development of advanced therapies

Methodologies cDNA cloning and mutagenesis RNA interfrence PCR and Real-time PCR Nuclear, cytoplasmic and mitochondrial protein extraction Western blotting Histology Immunofluorescence In situ enzymatic assays Digital Image analysis Cell culture of primary cells from the retina and cell lines Cell transfection and transduction with viruses RNA in situ hybridization