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Laboratorio di ricerca

Lab. namePlant Pathology Lab 
Principal Investigator Emilio Stefani 
ComponentsEmilio Stefani  (Principal Investigator )
Irem Altin  (Dottorando )
Sai Shiva Krishna Prasad Vurukonda   (Dottorando )
Silvia Volpato  (Dottorando )
Pascal Noel Mekam  (Dottorando )
Enrico Biondi  (Collaboratore esterno )
Davide Giovanardi  (Collaboratore esterno )
DescriptionMolecular epidemiology of plant bacterial diseases. Development and implementation of detection protocols for plant pathogens. Analysis of seed for the detection of regulated and non-regulated bacteria. Development and implementation of microbial biocontrol agents. Numerical taxonomy of phytopathogenic bacteria.  
Addressvia J. F. Kennedy, 17 - 42124 Reggio Emilia 
KeywordsPhytopathogenic bacteria, Detection, Disease control, Seed analysis, Biopesticides 
ERC Sectors LS1_1 Molecular interactions LS6_9 Bacteriology LS8_10 Microbial ecology and evolution LS9_4 Plant sciences (including crop production, plant breeding, agroecology, soil biology)  
InstrumentationIncubators, Sterile hoods, Microcentrifuges, Thermocyclers, Hybridation oven, Electrophoresis cells (vertical and horizontal). Immunofluorescence microscope. Growth chambers. 
Research Lines Plant, animal and microbial research for functional and sustainable food

Methodologies Isolation, purification and characterisation of plant pathogenic bacteria. Extraction, purification and amplification of microbial DNA and RNA. Multilocus sequence typing to assess bacterial phylogeny. In vitro and in planta assays to assess bacterial antagonism. Transformation of bacteria and their colonisation in plants assessed by confocal microscopy. Immunofluorescence antibody staining for the detection of phytopathogenic bacteria. Field trials to assess the efficacy of microbial biocontrol agents.