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Laboratorio di ricerca

Lab. nameUMCC Lab 
Principal Investigator Maria Gullo 
ComponentsMaria Gullo  (Principal Investigator - Coordinatore Scientifico UMCC )
Luciana De Vero  (Collaboratore - Curatore della collezione UMCC )
DescriptionUMCC Reseach Group harbours competences on functional starter cultures for food and industrial fermentation processes. These competances are supported by a combination of knowledge from several scientific disciplines including: taxonomy, analytical chemistry, ecology, biology, genetic engineering, fermentation, and informatics. UMCC Group performes field experiments, isolation and identification of microorganisms, qualitative and quantitative studies of microbial functionalities by modeling expertise. The UMCC Biobank holds about 2700 strains (yeasts, acetic acid bacteria and lactic acid bacteria), maintained in collection by techniques that ensure preservation and genetic stability for long term.The strains in the collection are characterized by polyphasic approach and all the information is carefully managed and monitored by BioloMICS NET software (BioAware).  
Addressvia J. F. Kennedy, 17 - 42124 Reggio Emilia 
KeywordsMicrobiology, Genomic Functional , UMCC, biobank, database BioloMICS, MIRRI-IT 
ERC Sectors 
InstrumentationTechnical platforms of the UMCC group include basic and specialized facilities for microbiology and molecular biotechnology: pilot bioreactor, laboratory fermentors, analytical equipments, bioinformatics softwares (BioloMICS and BIONUMERICS) for data management, sterilization systems (autoclaves and filtration systems), Singer micromanipulator, Biological cabinets (class 2), thermostated incubators, shaking incubators, oximeter, HPLC, sonicator, refrigerated centrifuges, PCR machines, electrophoretic sysems, 2-dimensional electrophoresis machine, PFGE (pulsied field gel electrophoresis), ultrafreezer -80°C, freezer -20°C.  
Research Lines Agrifood and Environment

Methodologies Genetic improvement of industrial and food-grade microorganisms. Fermentative test, growth and survival performance in function of different biotic and abiotic factors, metabolites detection by HPLC. Culture-independent technique (PCR-DGGE) for the microbioma characterization in complex matrices. Identification, characterization and molecular typing of yeasts: rapid screening and tentative species attribution by RFLP of the rDNA 5,8S-ITS region; Sequencing of D1/D2 region of the 26S rDNA gene. DNA fingerprinting and biotyping by: inter-delta analysis of TY1 e TY2 transposons elements for Saccharomyces cerevisiae strains; RAPD-PCR; analysis of microsatellites (GTG)5 e (GACA)4. Tetrad dissection and yeast hybrid construction by micromanipulator; Yeast transformation and cloning; gene expression profile by quantitative PCR. Yeast karyotyping by PFGE (pulsied field gel electrophoresis). Yeast DNA content by FACS; mitotype characterzation in yeasts; Southern and Northen blotting. Identification, characterization and molecular typing of acetic acid bacteria and lactic acid bacteria; partial and complete sequencing of 16S rDNA, 16S-23S (ITS) and phylogenetic analysis; ERIC/PCR, PCR/REP with single primer (GTG)5. Lactic acid bacteria cloning and transformation. PFGE (pulsied field gel electrophoresis). Targeted preservation procedures for slow growing strains. Selected starter cultures design and development. 
Web Site www.umcc.unimore.it/; www.mirri-it.it/