Gruppo di ricerca
Lab. name | Laboratory of genome editing and gene transfer |
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Principal Investigator | Alessandra Recchia |
Components | Alessandra Recchia (Principal Investigator
)
Daniela Benati (Collaboratore ) Eleonora Cattin (Collaboratore ) Tommaso Ferrari (Collaboratore ) |
Description | Main activities are: development of genome editing approaches to correct genetic defects using viral vectors (AAV, adenovirus and lentivirus) and non viral vectors. Molecular and genomic analysis to assess the editing and the genotoxicity. |
Address | via Glauco Gottardi, 100 - 41125 Modena |
Keywords | genome editing; genetic defects; viral and non viral vectors |
ERC Sectors | LS2_2 Gene editing LS2_14 Genetic diseases LS7_2 Medical technologies and tools (including genetic tools and biomarkers) for prevention, diagnosis, monitoring and treatment of diseases LS9_2 Applied genetics, gene editing and transgenic organisms |
Instrumentation | confocal microscopy (LSM 510 META, Zeiss) and related image acquisition apparatus; laser micro-dissector (PALM MICROBEAM, Zeiss); FACS (BD FACS CANTO II, BD); conventional PCR termocycler and RT-CR system (7900HT Fast Real Time PCR System, Apply Biosystems); Luminometer and ELISA reader (Glomax 96 Microplate Luminometer, Promega); NucleofectorTM device (Amaxa); Nanodrop® ND-1000 UV-Vis Spectrophotometer; tissue culture BL2 room equipped with: 3 laminar flow hoods, 2 CO2 incubators, 1 refrigerated supercentrifuge, 2 microscopes. |
Research Lines | Translational Medicine |
Methodologies | Genome editing with engineered nucleases e.g. ZFN , TALEN and CRISPR/Cas9. Development and production of AAV , Adenoviral and lentiviral vectors for research uses |
Web Site | @Recchia_Lab |