Gruppo di ricerca
Lab. name | Molecular Genetics |
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Principal Investigator | Carol Imbriano |
Components | Carol Imbriano (Principal Investigator
)
Virginia Campani (Dottorando ) Silvia Belluti (Ricercatore ) Valentina Mularoni (Assegnista ) Laura Cuoghi (Assegnista ) |
Description | TRANSCRIPTIONAL AND NON-TRANSCRIPTIONAL CONTROL OF CELL PROLIFERATION AND DIFFERENTIATION. The activity of the laboratory is focused on NF-Y, a heterotrimeric transcription factor with a “pioneering” role in the establishment of transcriptional programs. NF-Y regulates the transcription of several genes, such as cell cycle, pro- and anti-apoptotic genes, whose expression is fundamental in the balance between cell proliferation and cell death. NF-Y is one of the transcription factors orchestrating oncogenic transcriptional changes, and clinical studies correlated upregulated expression of its target genes to poor clinical prognosis in multiple types of cancer. Moreover, NF-Y has a key role in the control of proliferation and differentiation programs of pluripotent and adult stem cells. Our research is directed towards investigating the transcriptional and non-transcriptional activity of NF-Y and its splicing variants in cancer cells and muscle stem cells. In particular, we are interested in the identification of the specific transcriptional role of NF-Y splice isoforms that are differently expressed in tumor tissues compared to healthy ones. |
Address | via Giuseppe Campi, 213/d - 41125 Modena |
Keywords | Gene transcription, alternative splicing, cancer cell proliferation, muscle stem cells |
ERC Sectors | LS2_1 Genetics LS2_3 Epigenetics LS2_4 Gene regulation LS3_1 Cell cycle, cell division and growth LS3_13 Stem cells |
Instrumentation | qPCRs, protein and DNA analysis instrumentations, electroporator, benchtop and refrigerated centrifuges, coldbox, Amersham imager |
Research Lines | Translational Medicine |
Methodologies | 2D and 3D cell cultures, chromatin immunoprecipitation, DNA cloning, nucleic acids extraction, DNA and RNA analysis, protein analysis (electrophoresis and western blot), immunofluorescence, protein-protein interactions, cytofluorimetric cell cycle analysis, generation of transgenic mouse models, in vivo analysis of muscle stem cells |