Laboratorio di ricerca
| Lab. name | BioPaCT - Biology and Pathology of soft Connective Tissues |
|---|---|
| Principal Investigator | Daniela Quaglino |
| Components | Daniela Quaglino (Principal Investigator
)
Francesco Demetrio Lofaro (Dottorando ) Federica Boraldi (Ricercatore ) |
| Description | The biology and pathology of the extracellular matrix is investigated looking at 1) structural and metabolic alterations occurring in skin and in blood vessels during aging, in acquired and in genetic diseases, 2) DNA sequencing and molecular pathways leading to ectopic calcifications (ie. PXE and PXE-like disorders), 3) protein profiling of human fibroblasts in different experimental conditions, 4) structural and biological characterization of elastin and elastin-derived peptides. |
| Address | via Giuseppe Campi, 287 - 41125 Modena |
| Keywords | Extracellular matrix, aging, ectopic calcification, connective tissue genetic diseases, elastin |
| ERC Sectors | LS4_1 Organ physiology and pathophysiology LS4_1 Organ physiology and pathophysiology LS4_4 Ageing LS4_5 Metabolism, biological basis of metabolism related disorders LS4_5 Metabolism, biological basis of metabolism related disorders LS4_5 Metabolism, biological basis of metabolism related disorders |
| Instrumentation | PCR Thermal cyclers, -80C Freezers , IpgPhor IEF and Ettan Dalt 6 for 2D-IPG, Image Scanner II, Zeiss Axiophot microscope, ultramicrotomes, centrifuges, chemical hoods Other major instruments (i.e. DNA sequencing, electron and confocal microscopy, mass spectrometry) are available at Inter-department facilities. |
| Research Lines | Translational Medicine |
| Methodologies | Structural and ultrastructural analyses by light and fluorescence microscopy, confocal microscopy, transmission and scanning electron microscopy; immunocytochemistry and immunoelectronmicroscopy; protein and proteome analysis associated with MS/MS; PCR and DNA sequencing, cell culture, biocompatibility tests. |