Laboratorio di ricerca
| Lab. name | Molecular Genetics |
|---|---|
| Principal Investigator | Carol Imbriano |
| Components | Carol Imbriano (Principal Investigator
)
Silvia Belluti (Assegnista ) Giovanna Rigillo (Assegnista ) |
| Description | TRANSCRIPTIONAL AND NON-TRANSCRIPTIONAL CONTROL OF CELL PROLIFERATION AND DIFFERENTIATION. The activity of the laboratory is focused on NF-Y, a heterotrimeric transcription factor with a “pioneering” role in the establishment of transcriptional programs. NF-Y regulates the transcription of several genes, such as cell cycle, pro- and anti-apoptotic genes, whose expression is fundamental in the balance between cell proliferation and cell death. NF-Y is one of the transcription factors orchestrating oncogenic transcriptional changes, and clinical studies correlated upregulated expression of its target genes to poor clinical prognosis in multiple types of cancer. Moreover, NF-Y has a key role in the control of proliferation and differentiation programs of pluripotent and adult stem cells. Our research is directed towards investigating the transcriptional and non-transcriptional activity of NF-Y and its splicing variants in cancer cells and muscle stem cells. |
| Address | via Giuseppe Campi, 213/d - 41125 Modena |
| Keywords | Gene transcription, alternative splicing, cancer cell proliferation, muscle stem cells |
| ERC Sectors | LS2_6 Molecular genetics, reverse genetics and RNAi LS2_8 Epigenetics and gene regulation LS3_5 Cell differentiation, physiology and dynamics LS3_12 Stem cell biology LS4_6 Cancer and its biological basis |
| Instrumentation | qPCRs, protein and DNA analysis instrumentations, electroporator, benchtop and refrigerated centrifuges, coldbox, Amersham imager |
| Research Lines | Translational Medicine |
| Methodologies | 2D and 3D cell cultures, chromatin immunoprecipitation, DNA cloning, nucleic acids extraction, DNA and RNA analysis, protein analysis (electrophoresis and western blot), immunofluorescence, protein-protein interactions, cytofluorimetric cell cycle analysis, generation of transgenic mouse models, in vivo analysis of muscle stem cells |