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Laboratorio di ricerca

Lab. nameMolecular Genetics 
Principal Investigator Carol Imbriano 
ComponentsCarol Imbriano  (Principal Investigator )
Silvia Belluti  (Assegnista )
Giovanna Rigillo  (Assegnista )
DescriptionTRANSCRIPTIONAL AND NON-TRANSCRIPTIONAL CONTROL OF CELL PROLIFERATION AND DIFFERENTIATION. The activity of the laboratory is focused on NF-Y, a heterotrimeric transcription factor with a “pioneering” role in the establishment of transcriptional programs. NF-Y regulates the transcription of several genes, such as cell cycle, pro- and anti-apoptotic genes, whose expression is fundamental in the balance between cell proliferation and cell death. NF-Y is one of the transcription factors orchestrating oncogenic transcriptional changes, and clinical studies correlated upregulated expression of its target genes to poor clinical prognosis in multiple types of cancer. Moreover, NF-Y has a key role in the control of proliferation and differentiation programs of pluripotent and adult stem cells. Our research is directed towards investigating the transcriptional and non-transcriptional activity of NF-Y and its splicing variants in cancer cells and muscle stem cells.  
Addressvia Giuseppe Campi, 213/d - 41125 Modena 
KeywordsGene transcription, alternative splicing, cancer cell proliferation, muscle stem cells 
ERC Sectors LS2_6 Molecular genetics, reverse genetics and RNAi LS2_8 Epigenetics and gene regulation LS3_5 Cell differentiation, physiology and dynamics LS3_12 Stem cell biology LS4_6 Cancer and its biological basis  
InstrumentationqPCRs, protein and DNA analysis instrumentations, electroporator, benchtop and refrigerated centrifuges, coldbox, Amersham imager 
Research Lines Translational Medicine

Methodologies 2D and 3D cell cultures, chromatin immunoprecipitation, DNA cloning, nucleic acids extraction, DNA and RNA analysis, protein analysis (electrophoresis and western blot), immunofluorescence, protein-protein interactions, cytofluorimetric cell cycle analysis, generation of transgenic mouse models, in vivo analysis of muscle stem cells