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Laboratorio di ricerca

Lab. nameLactic acid bacteria and Yeast Biotechnology (LYB) 
Principal Investigator Lisa Solieri 
ComponentsLisa Solieri  (Principal Investigator - Asscociate Professor )
DescriptionThe research of the Lactic acid bacteria and Yeast Biotechnology (LYB) lab deals with the quantitative and qualitative study of the community dynamics, species diversity, and metabolomics of fermented food ecosystems, with special regards to yeasts and lactic acid bacteria populations. The ultimate purpose of LYB's research is to understand why given microorganisms prevail in specific food ecosystems and which molecular mechanisms are responsible for competitiveness and functionality traits. This body of knowledge drives the development of novel cell factories for controlled and sustainable bio-process, as well as of healthy food products. In detail, we are currently working on three main topics: • Development of Saccharomyces and non-Saccharomyces yeast cell factories for food and industrial bio-processes by GMO and non-GMO techniques. • Isolation, identification and physiological characterization of novel food-grade lactic acid bacteria cultures with healthy functionalities from sourdough and dairy ecosystems. • Study of genetic systems underlying sexual reproduction, mating, and cell-to-cell adhesion in order to unravel crucial yeast evolutionary mechanisms exploitable in GMO-free development of novel food-grade yeast cultures.  
Addressvia J. F. Kennedy, 17 - 42124 Reggio Emilia 
Keywordsapplied microbiology; healthy microbial cultures; food yeasts 
ERC Sectors LS2_1 Genomics, comparative genomics, functional genomics LS6_7 Microbiology LS8_3 Systems evolution, biological adaptation, phylogenetics, systematics, comparative biology LS8_10 Microbial ecology and evolution LS9_1 Non-medical biotechnology and genetic engineering (including transgenic organisms, recombinant proteins, biosensors, bioreactors, microbiology) LS9_5 Food sciences (including food technology, nutrition)  
InstrumentationBasic microbiology: Laminar flow, autoclave, ULT freezer, stomacher, dry ovens, centrifuges, incubators, (water bath) shakers, water baths, cell disrupter (sonicator), spectrophotometers, microscope. Molecular biology: Thermocycler, 7300 real-time PCR system (Applied Biosystems), standard electrophoresis equipment (nucleic acids, proteins), Nanodrop ND-2000 (Thermo Fisher Scientific), DGGE apparatus.  
Research Lines Agrifood and Environment

Methodologies General microbiology techniques for cultivation of yeast and lactic acid bacteria. General molecular biology techniques (DNA cloning and expression, PCR, electrophoresis of DNA, RNA, genotyping, Sanger sequencing); transformation and heterologous protein expression. Culture independent techniques for description of microbial community (DGGE, q-PCR, analysis of metagenome datasets).